BCAR3 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA
Recommend Dilution	ELISA: 1:100-1:2000
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	DNA immunization. This antibody is specific for the Middle Region of the target protein.
Isotype	IgG
Formulation	20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Concentration	1.3mg/ml
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	BCAR3 adaptor protein, NSP family member
Database Link	NP_003558 Entrez Gene 8412 Human UniProt ID O75815
Background	In breast cancer, the antiestrogen tamoxifen has been prescribed for both primary treatment and the treatment of advanced metastatic disease. Although the drug induces remission in most patients with estrogen receptor-positive disease, all patients eventually develop resistance. van Agthoven et al. (1998) applied an insertional mutagenesis strategy to a breast cancer cell line. Infected cells were subjected to tamoxifen selection, and the resistant clones were screened for a common integration site linked with antiestrogen resistance. By screening a human testis cDNA library with the integration site-specific probe, they obtained a cDNA encoding BCAR3 (breast cancer antiestrogen resistance 3). The deduced 825 amino acid BCAR3 protein contains a putative Src homology 2 (SH2) domain and sequences homologous to yeast CDC48. Northern blot analysis detected abundant expression of a 3.4kb BCAR3 transcript in heart, placenta, skeletal muscle, spleen, prostate, testis, ovary, small intestine, colon, fetal kidney, and several cancer cell lines, but not in nonmalignant breast tissue; a 6kb BCAR3 transcript was also detected in skeletal muscle and heart.
Synonyms	NSP2; SH2D3B
Note	This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
Reference Data
Protein Families	Druggable Genome

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