EIF4E Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI6C3]

Specifications

Product Data
Clone Name	OTI6C3
Applications	FC, IHC, WB
Recommend Dilution	WB 1:500, IHC 1:150
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Full length human recombinant protein of human EIF4E (NP_001959) produced in HEK293T cell.
Isotype	IgG1
Formulation	PBS (pH 7.3) containing 1% BSA, 50% glycerol.
Concentration	0.5 mg/ml
Purification	Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation	HRP
Storage Condition	Store at -20°C as received.
Predicted Protein Size	24.9 kDa
Gene Name	eukaryotic translation initiation factor 4E
Database Link	NP_001959 Entrez Gene 13684 MouseEntrez Gene 117045 RatEntrez Gene 1977 Human UniProt ID P06730
Background	All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G (MIM 600495) (Rychlik et al., 1987 [PubMed 3469651]). [supplied by OMIM]
Synonyms	AUTS19; CBP; eIF-4E; EIF4E1; EIF4EL1; EIF4F
Reference Data
Protein Pathways	Insulin signaling pathway, mTOR signaling pathway

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