CDH1 Rabbit Monoclonal Antibody [Clone ID: 19A11]

Specifications

Product Data
Clone Name	19A11
Applications	ELISA, FC, WB
Reactivity	Human, Dog
Modifications	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Host	Rabbit
Clonality	Monoclonal
Immunogen	The original antibody was generated by immunizing mice with purified ECad1-5 protein.
Specificity	This antibody recognizes the N-terminal extracellular cadherin repeat domain 1 (EC1) of the human E-cadherin. This antibody also cross reacts with canine E-cadherin. Cadherins are calcium-dependent cell adhesion proteins. It is localized on the surfaces of epithelial cells in regions of cell-cell contact known as adherens junctions. E-cadherins are reported to play an important role in cancer metastasis. The initial screeding of E-Cadherin–positive hybridoma supernatants for binding to live Colo 205 cells was done using an ELISA. Activating antibody 19A11 stimulated the adhesion of Colo 205 cells to pure E-cadherin substrate (PMID: 22513089). This antibody is also capable of recognizing denature human E-cadherin in a western blot. This antibody also inhibited the formation of epithelial tubules, causing the cells to remain as round epithelial cysts. This antibody also enhanced the adhesion of HGF-treated MDCK cells to E-cadherin when analyzed with the flow adhesion assay (PMID: 22513089). This antibody slowed the rate of wound closure in both normal culture medium and HGF-treated cultures (PMID: 22513089). This antibody is an E-cadherin activating antibody which stimulates the apoptosis of circulating tumor cells (PMID: 32127478). This antibody was used in determination of expression of E-cadherin on low-adherent Colo205 cells using flow cytometry (PMID: 27582386).
Isotype	IgG, kappa
Formulation	PBS with 0.02% Proclin 300.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Database Link	UniProt ID P12830
Synonyms	Cadherin-1; Cadherin E; CAM 120/80; CD324; E-Cadherin; epithelial cadherin; Uvomorulin
Reference Data

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