Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	WB: 1:1,000-1:5,000 ELISA: 1:10,000-1:50,000
Host	Rabbit
Clonality	Polyclonal
Immunogen	This antibody was purified from whole rabbit serum prepared by repeated immunizations with the MBP epitope tag recombinant protein.
Specificity	This IgG purified antibody is directed against MBP and is useful in determining its presence in various assays. This polyclonal anti-MBP tag antibody detects over-expressed proteins containing the MBP epitope tag. To date this antibody has reacted with all MBP tagged proteins so far tested. In western blotting of bacterial extracts the antibody does not cross-react with endogenous proteins.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	1.0 mg/mL - lot specific
Conjugation	Biotin
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Background	Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. Rockland Immunochemicals produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Rockland Immunochemicals also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.
Synonyms	rabbit anti-MBP Epitope Tag Antibody biotin conjugation, rabbit anti-Maltose Binding Protein Antibody biotin conjugated
Note	Anti-MBP Biotin Conjugated Antibody is optimally suited for monitoring the expression of MBP tagged fusion proteins. As such, anti- MBP/MBP can be used to identify fusion proteins containing the MBP epitope. The antibody recognizes the MBP epitope tag fused to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against MBP containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry, and other immunodetection techniques. Maltose binding protein is a bacterial protein, which is often used in protein expression studies because it creates a stable fusion product that does not appear to interfere with the bioactivity of the protein of interest. It also allows for its easy purification from bacterial extracts under mild conditions. Anti-MBP is a companion to the pMAL protein expression system and can be used for the detection and purification of MBP-fusion proteins expressed in E. coli. By Western blot, a band is seen at ~ 42 kDa representing MBP.
Reference Data

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