IL8 (CXCL8) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, IP, WB
Recommend Dilution	WB: 1:500 - 1:2,000 IHC: 1:400 - 1:800 ELISA: 1:1,000 - 1:5,000
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	The whole rabbit serum was prepared by repeated immunizations with recombinant human IL-8 produced in E.coli.
Specificity	Anti-IL 8 antiserum has been heated to 56°C for 30 minutes.  The antiserum detects recombinant and native IL-8 present in body fluids and cell supernatants in ELISA.  Although likely to be functional, this antiserum has not been evaluated in other assays, including neutralization and immunohistochemistry.
Formulation	None
Concentration	75 mg/mL - lot specific
Conjugation	Unconjugated
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Gene Name	C-X-C motif chemokine ligand 8
Database Link	Entrez Gene 3576 Human UniProt ID P10145
Background	Interleukin-8 is a chemokine produced by macrophages and other cell types such as epithelial cells. Human IL-8 (CXCL8) antibody is commonly used in many experiments focused around inflammation. Anti-IL-8 antibody is ideal for investigators involved in Cancer, Immunology and Cardiovascular research.
Synonyms	Anti-CXCL 8, CXCL8 antibody, 310C antibody, AMCF 1 antibody, AMCF1 antibody, b ENAP antibody, Beta thromboglobulin like protein antibody, CXC chemokine ligand 8 antibody, nterleukin8 antibody, K60 antibody, LECT antibody, LUCT antibody, rabbit anti-Interleukin 8 Antibody, rabbit anti-IL-8
Note	IL 8 antibody has been tested for use in ELISA and western blot. It recognizes IL-8 found in cells, supernatants and other body fluids.  Reactivity in other immunoassays is unknown.
Reference Data

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