Angpt1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	WB: 1:500-1:2,000 IHC: 1:200-1:800 ELISA: 1:5,000 - 1:25,000
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to a N-terminus region near aa 15-45 of mouse angiopoietin 1 protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.
Specificity	Anti-Angiopoietin 1 is directed against mouse angiopoietin-1 and shows no reactivity with mouse angiopoietin-2. This reagent cross-reacts with human angiopoietin 1. Partially cross reactivity is noted with human angiopoietin 2. This product was prepared from monospecific antiserum by a delipidation and defibrination.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	80 mg/ml - lot specific
Conjugation	Unconjugated
Storage Condition	Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Gene Name	angiopoietin 1
Database Link	Entrez Gene 11600 Mouse UniProt ID O08538
Background	Anti-Antiopoietin-1 Antibody, which recognizes Angiopoietin-1 (Ang-1), has importance in the development of the endothelium by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has a potential fibrinogen-like domain at the carboxyl terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, Ang-1 is also expressed in the heart and liver. Anti-Antiopoietin-1 Antiserum antibody is suitable for cardiovascular research.
Synonyms	rabbit anti-Angiopoietin 1 Antibody, rabbit anti-ANG1 antibody, AGP 1 antibody, AGP1 antibody, AGPT antibody, ANG 1 antibody, Angiopoietin1 antibody, ANGPT 1 antibody, ANGPT1 antibody, KIAA0003 antibody
Note	Anti-Antiopoietin-1 Antiserum has been tested in ELISA, western blot, immunohistochemistry, and immunofluorescence and is suitable for other antibody based assays.  A 1:500 dilution is recommended for western blotting.  The reaction of this antiserum directly with cell supernatants may result in high background due to reactivity of components in the serum.  This can be alleviated by first immunoprecipitating the antibody:antigen complex and then detecting the antigen. This method results in a very clean and strong signal.  Both Ang-1 and Ang-2 proteins have predicted molecular weights of approximately 57 kDa and appear on western blots close to their predicted molecular weights.  In some instances additional bands may be seen at approximately 75 kDa which represent highly glycosylated forms of the protein that migrate at a higher apparent molecular weight.
Reference Data

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