Phospho Akt (Thr450) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB: 1:500~1:1000 IHC: 1:50~1:200
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Threonine 450.
Specificity	p-AKT (T450) polyclonal antibody detects endogenous levels of AKT1 only when phosphorylated at Thr450. This antibody also recognizes AKT2 and AKT3 when phosphorylated at the corresponding residues.
Isotype	IgG
Formulation	Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Concentration	1mg/ml
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Predicted Protein Size	~ 60 kDa
Database Link	UniProt ID P31749/P31751/Q9Y243
Background	AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer.
Synonyms	AKT1; AKT2; AKT3; PKB; PKB alpha; PKB beta; PKBG; PKB gamma; PKBα; PKBβ; PKBγ; Protein kinase Akt-2; Protein kinase Akt-3; Protein kinase B; Protein kinase B alpha; Protein kinase B beta; Protein kinase B gamma; Proto-oncogene c-Akt; RAC; RAC-alpha serine/threonine-protein kinase; RAC-beta serine/threonine-protein kinase; RAC-gamma serine/threonine-protein kinase; RAC-PK-alpha; RAC-PK-beta; RAC-PK-gamma; STK-2
Note	For research use only, not for use in diagnostic procedure.
Reference Data

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