SMAD2 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB: 1:1000~1:2000
Reactivity	Human, Rat, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide, corresponding to Human Smad2.
Specificity	Smad2 (Thr220) polyclonal antibody detects endogenous levels of Smad2 protein.
Isotype	IgG
Formulation	Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Concentration	1mg/ml
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Predicted Protein Size	~ 53 kDa
Gene Name	SMAD family member 2
Database Link	Entrez Gene 4087 Human UniProt ID Q15796
Background	Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7. Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy-terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses.
Synonyms	hMAD-2; hSMAD2; JV18-1; Mad-related protein 2; MADH2, MADR2; MAD homolog 2; Mothers against decapentaplegic homolog 2; Mothers against DPP homolog 2; SMAD 2; SMAD2; Smad2; SMAD family member 2
Note	For research use only, not for use in diagnostic procedure.
Reference Data

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