RAF1 Mouse Antibody [Clone ID: M208]

Specifications

Product Data
Clone Name	M208
Applications	WB
Recommend Dilution	WB: 1:1000
Reactivity	Human, Rat, Mouse
Host	Mouse
Immunogen	Clone (M208) was generated from a sequence corresponding to amino acids in the N-terminal region of human C-Raf. This C-Raf sequence has high homology to rat and mouse C-Raf.
Specificity	This antibody detects a 74 kDa* protein corresponding to the molecular mass of C-Raf on SDS-PAGE immunoblots of human A431 and Jurkat cells.
Formulation	PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Concentration	lot specific
Purification	Protein G Purified
Conjugation	Unconjugated
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Predicted Protein Size	74
Database Link	UniProt ID P04049
Background	The Ras-Raf-MAP kinase signaling pathway is involved in control of cell proliferation and differentiation. The Raf kinase family includes A-Raf, B-Raf, and C-Raf. Each family member has three highly conserved regions (CR1-3). The N-terminal CR1 contains the Ras-GTP-binding domain. The CR2 contains a negative regulatory serine residue (C-Raf (S259)/B-Raf(S365)) that may bind 14-3-3 proteins. The CR3 is the catalytic domain that contains phosphorylation sites for Raf-regulating enzymes within two segments, the N-region and the activation segment. Activation of C-Raf involves phosphorylation at many sites including Ser-338, Tyr-341, and multiple catalytic domain sites. EGF receptor activation leads to phosphorylation of Ser-471, which is critical for C-Raf kinase activity and is required for interaction with MEK. In B-Raf, the corresponding conserved site is Ser-578, and mutation of this residue to alanine produces an inactivate kinase. Thus, this Raf phosphorylation site may be critical for kinase activity and may be important for MEK binding and activation
Note	Protein G purified tissue culture supernatant.
Reference Data

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