Phospho-Phospho (Ser/Thr) (pSer/Thr) Mouse Monoclonal Antibody [Clone ID: M380A/M380B]

Specifications

Product Data
Clone Name	M380A/M380B
Applications	ICC, IP, WB
Recommend Dilution	WB: 1:500 ICC: 1:50
Reactivity	Human, Rat, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	PM3801 Phosphoserine/threonine is a mix of two clones: Clone M380A was generated from a phosphothreonine synthetic peptide (coupled to carrier protein) and Clone M380B was generated.from a phosphoserine synthetic peptide (coupled to carrier protein).
Specificity	The antibody detects many serine or threonine phosphorylated proteins by western blot, immunocytochemistry, and ELISA.
Isotype	IgG1
Formulation	PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Concentration	lot specific
Purification	Protein A Purified
Conjugation	Unconjugated
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Background	Phosphorylation of specific serine or threonine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor ser/thr kinases that mediate these protein modifications. Antibodies that can detect phosphoserine or phosphothreonine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphorylated proteins. Immunoprecipitation of proteins of interest followed by detection of phosphoserine or phosphothreonine using anti-phosphoserine antibody is commonly used to correlate changes in phosphorylation state with alterations in protein activity.
Note	Protein G purified tissue culture supernatant.
Reference Data

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