CDKN1A Mouse Antibody [Clone ID: M513]
CNY 3413.00
货期*
5周
规格
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Specifications
Product Data | |
Clone Name | M513 |
Applications | ICC, IHC, IP, WB |
Recommend Dilution | WB: 1:500 ICC: 1:50 |
Reactivity | Human |
Host | Mouse |
Immunogen | Clone M513 was generated from a full length recombinant human p21 protein. The antibody detects human p21. |
Specificity | The antibody detects a 21 kDa* protein corresponding to the apparent molecular mass of p21 on SDS-PAGE immunoblots of human endothelial cells (HUVEC), HeLa cells, and Fibroblasts. The antibody is also useful for immunoprecipitation and immunofluorescent labeling in human cells. |
Isotype | IgG2a |
Formulation | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
Concentration | lot specific |
Purification | Antigen Affinity Purified |
Conjugation | Unconjugated |
Storage Condition | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
Predicted Protein Size | 21 |
Database Link | |
Background | The tumor suppressor protein p21/CIP1/WAF1 acts as an inhibitor of cell cycle progression. It functions in stoichiometric relationships forming heterotrimeric complexes with cyclins and cyclin-dependent kinases. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S. However, p21 may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D. The carboxy-terminal region of p21 is sufficient to bind and inhibit PCNA, a subunit of DNA polymerase, and may coordinate DNA replication with cell cycle progression. Upon UV damage or during cell cycle stages when cdc2/cyclin B or CDK2/cyclin A are active, p53 is phosphorylated and upregulates p21 transcription via a p53-responsive element. Protein levels of p21 are downregulated through ubiquitination and proteasomal degradation. |
Note | Protein G purified tissue culture supernatant. |
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