BSG Mouse Antibody [Clone ID: M052]

Specifications

Product Data
Clone Name	M052
Applications	WB
Recommend Dilution	WB: 1:2000
Reactivity	Human
Host	Mouse
Immunogen	Clone (M052) was generated from a proprietary antigen related to the native human CD147 expressed in NCI-H1915 lung cancer cell line.
Specificity	Clone M052 mouse monoclonal antibody detects a 38-58 kDa* protein on SDS-PAGE immunoblots of human SK-MES1, MDA-MB-231, MeWo, A431, LNCaP, and NCI-H1915 cells, as well as human brain tissues. The antibody works for western blot and ELISA detection of CD147.
Isotype	IgG1
Formulation	PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Concentration	lot specific
Purification	Protein G Purified
Conjugation	Unconjugated
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Predicted Protein Size	38-58
Database Link	UniProt ID P35613
Background	D147 (Basigin/EMMPRIN) is a type I integral membrane receptor protein belonging to the immunoglobulin superfamily. CD147 is a glycosylated protein with four known isoforms with isoform 2 being the most abundantly expressed. In many cancers, CD147 is overexpressed and stimulates the secretion of extracellular matrix metalloproteinases by tumor fibroblasts, which can promote tumor progression. CD147 is co-expressed with the amino acid transporter, CD98h, in metabolically active cells such as lymphocytes, macrophages, and tumor cells. CD147 interaction with caveolin and cyclophilins regulates its functions, and this protein may function outside of tumor cells through cell shedding and microvesicle release.
Note	Protein G purified tissue culture supernatant.
Reference Data

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