CD276 Mouse Antibody [Clone ID: M045]

Specifications

Product Data
Clone Name	M045
Applications	ICC, IP, WB
Recommend Dilution	WB: 1:1000 ICC: 1:100
Reactivity	Human
Host	Mouse
Immunogen	Clone (M045) was generated from a recombinant protein that included the extracellular region of human B7-H3 protein.
Specificity	Clone M045 mouse monoclonal antibody detects a 100-120 kDa* protein on SDS-PAGE "Native" or denatured immunoblots of human LNCaP, MCF-7, NCI-H28, A431, and MDA-MB-231 carcinomas. The antibody detects B7-H3 in membranes and cytoplasm in MCF7 cell after immunocytochemical labeling. The antibody works for western blot, immunoprecipitation, immunocytochemistry, and ELISA capture.
Isotype	IgG1
Formulation	PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Concentration	lot specific
Purification	Protein G Purified
Conjugation	Unconjugated
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Predicted Protein Size	100-120
Database Link	UniProt ID Q5ZPR3
Background	B7 homolog 3 (B7-H3, CD276) is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses. B7-H3 is a membrane protein with an extracellular region that includes two Ig-like V-type domains and two IgG-like C2-type domains, and a short intracellular domain. B7-H3 is a regulatory molecule for immune reactions, such as T cell proliferation and IFN-γ production. In colon cancers, B7-H3 inhibits T-cell cytotoxicity, and blocking B7-H3 function enhances T-cell cytotoxicity toward cancer cells. B7-H3 is expressed by antigen presenting cells, activated T cells, and a few normal tissues, including placenta and prostate. In cancer, B7-H3 is expressed in various tumor types including prostate, breast, colon, lung, and gastric cancers. The B7-H3 expression level correlates with tumor growth, invasion, metastasis, malignant stage, and recurrence rate. The inhibition or blockade of B7-H3 function could be an important immunotherapeutic approach for several types of cancer
Note	Protein G purified tissue culture supernatant.
Reference Data

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