Ki67 (MKI67) Mouse Monoclonal Antibody [Clone ID: 6G3]

Specifications

Product Data
Clone Name	6G3
Applications	ICC, IHC, WB
Recommend Dilution	WB: 1:2000 ICC: 1:2000-1:5000
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant construct containing the N-terminal 300 amino acids of the human sequence expressed in and purified from E. coli.
Specificity	Specific for endogenous levels of the ~345 & 395 kDa Ki-67 isoforms. Additional bands are thought to be breakdown products of these 2 isoforms.
Isotype	IgG1
Formulation	PBS + 50% glycerol and 5 mm NaN3
Concentration	lot specific
Purification	Protein G Purified
Conjugation	Unconjugated
Storage Condition	Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Predicted Protein Size	345-395
Gene Name	marker of proliferation Ki-67
Database Link	Entrez Gene 4288 Human UniProt ID P46013
Background	Ki-67 is a nuclear protein that is considered to be essential for cellular proliferation since it is expressed only in active phases of the cell cycle (G1, S, G2 and mitosis) but not in resting phase G0 (Scholzen & Gerdes 2000). Ki-67 antibodies have therefore been widely used as markers of cell growth. Ki-67 has further been identified as a predicative and prognostic marker in breast, lung, cervical and other cancers (Yerushalmi et al., 2010; Folescu et al., 2018; Das et al., 2018).
Synonyms	KI67; KIA
Note	Protein G purified culture supernatant
Reference Data

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