CSF3 Mouse Monoclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Sandwich ELISA: In a sandwich ELISA (assuming 100ul/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 100pg/ml of Recombinant Human G-CSF when used with ProSci’s Biotinylated Antigen Affinity Purified anti-Human G-CSF as the detection antibody, at a concentration of approximately 0.5- 1.0 μg/ml. Western Blot To detect Human G-CSF by Western Blot analysis, this antibody can be used at a concentration of 0.20-0.40 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human G-CSF is 1.5-2.0 ng/lane under non-reducing conditions, and 7.5-10.0 ng/lane under reducing conditions.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Produced in BALB/c mice immunized with highly pure Recombinant Human G-CSF. Anti-Human G-CSF-specific antibody was purified from cell culture by Protein A affinity chromatography.
Concentration	lot specific
Purification	G-CSF-specific antibody was purified from cell culture by Protein A affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Database Link	UniProt ID P09919
Background	G-CSF is a hematopoietic growth factor that stimulates the development of committed progenitor cells to neutrophils and enhances the functional activities of the mature end-cell. It is produced in response to specific stimulation by a variety of cells, including macrophages, fibroblasts, endothelial cells and bone marrow stroma. G-CSF is being used clinically to facilitate hematopoietic recovery after bone marrow transplantation. Human and murine G-CSF are cross-species reactive. Recombinant Human G-CSF is an 18.7 kDa protein consisting of 174 amino acid residues.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.