TSEN2 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Recommended dilution: WB:1:200-1:1000, IHC:1:50-1:200
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Recombinant Human tRNA-splicing endonuclease subunit Sen2 protein (1-220AA)
Isotype	IgG
Formulation	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration	lot specific
Purification	Antigen Affinity Purified
Conjugation	Unconjugated
Storage Condition	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Database Link	UniProt ID Q8NCE0
Background	Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Isoform 1 probably carries the active site for 5'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events. Isoform 2 is responsible for processing a yet unknown RNA substrate. The complex containing isoform 2 is not able to cleave pre-tRNAs properly, although it retains endonucleolytic activity.
Reference Data

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