CD32A (FCGR2A) Rabbit Monoclonal Antibody [Clone ID: IV.3]

Specifications

Product Data
Clone Name	IV.3
Applications	Bl, ELISA, IF, IP
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal
Immunogen	The original antibody was generated against the FcR of K562 cells.
Specificity	This antibody recognizes and binds CD32a. This antibody successfully precipitated a 40-kd FcR from lysates of U937 and K562 cells but not from Daudi or Raji cells. It selectively inhibited the binding of murine IgG1-coated erythrocytes to U937 cells. IV.3 blocked mIgG1 anti-T3 (Leu-4) stimulation but not mIgG2a anti-T3 (OKT3) stimulation of T cells (PMID: 2936805). Anti-FcyRII antibody IV.3 specifically stained Ltk- cells transfected with the FcyR cDNA clone in an immunofluorescence assay (PMID: 2824655). It was reported that IV.3 induces anaphylaxis in immunocompetent CD32a-transgenic “FCGR2A” mice and that IV.3 also induce severe thrombocytopenia in FCGR2A mice (PMID: 26396093). IV.3, reacted with CD32A-R131 exclusively, confirming the specificity in an ELISA (PMID:17386079).
Isotype	IgG, kappa
Formulation	PBS with 0.02% Proclin 300.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
Gene Name	Fc fragment of IgG receptor IIa
Database Link	Entrez Gene 2212 Human UniProt ID P12318
Synonyms	CD32; CD32A; CDw32; Fc-gamma-RIIa; FCG2; FcGR; FCGR2; FCGR2A1; FcRII-a; IGFR2; MGC23887; MGC30032
Note	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Reference Data

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