CD63 Rabbit Monoclonal Antibody [Clone ID: NK-1-C3]

Specifications

Product Data
Clone Name	NK-1-C3
Applications	FC, IF, IHC, IP, WB
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Monoclonal
Immunogen	This antibody was raised by immunizing BALB/c mice with a plasma membrane preparation of the human melanoma MeWo cells.
Specificity	This antibody recognizes CD63. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation and plays a role in the activation of cellular signaling cascades. The antibody NK-1-C3 has been widely used for the detection and analysis of CD63 via various methods. For instance, it was utilized as a marker of late endosomes and exosomes and the expression of CD63 was analysed via flow cytometry (Wang et al., 2019). Furthermore, NK-1-C3 (NK1/C3) proved successfull in labelling CD63 in multiple tissues, for example, when localizing CD63 in murine retinal pigment epithelium (RPE)/choroid tissue (Wang et al., 2009). Similarly, this antibody was used to detect CD63 on melanoma cells via immunofluorescence staining (Khazen et al., 2016).
Isotype	IgG, kappa
Formulation	PBS with 0.02% Proclin 300.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
Gene Name	CD63 molecule
Database Link	Entrez Gene 967 Human UniProt ID P08962
Synonyms	granulophysin; LAMP-3; ME491; MLA1; OMA81H; tetraspanin-30; Tspan-30; TSPAN30
Note	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Reference Data

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