CD11a (ITGAL) Rabbit Monoclonal Antibody [Clone ID: MHM24]

Specifications

Product Data
Clone Name	MHM24
Applications	Inhib
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal
Immunogen	Spleen cells from BALB/c mice immunised with EBV-transformed human lymphoblastoid cells were fused with P3-NSUlAg4.1 myeloma cells and the resulting hybridomas were screened for antibody production by a radioimmunoassay on T and B cell lines.
Specificity	MHM24 binds to the CD11a subunit of lymphocyte function-associated antigen. It binds to both the high- and low-affinity forms of the antigen. Cytofluorography was used to show the binding of MHM24 to peripheral blood lymphocytes, with 97.3% of cells stained by MHM24 (Hildreth et al, 1983). In inhibition experiments, MHM24 inhibited T cell-mediated lysis of virus-infected cells (Hildreth et al, 1983). Additionally, MHM24 inhibited NK cell-mediated lysis (Hildreth et al, 1983).Flow cytometry showed MHM24 exhibits non-selective binding to high-affinity and low-affinity forms of LFA-1 (Huang et al, 2006). Murine MHM24 was humanised and shown to prevent adhesion of human T cells to human keratinocytes and the proliferation of T cells in response to nonautologous leukocytes (Werther et al, 1996).
Isotype	IgG, kappa
Formulation	PBS with 0.02% Proclin 300.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
Gene Name	integrin subunit alpha L
Database Link	Entrez Gene 3683 Human UniProt ID P20701
Synonyms	CD11A; LFA-1; LFA-1A; LFA1A
Note	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.
Reference Data

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