EIF4E Rabbit Monoclonal Antibody [Clone ID: R04-5A4]

Specifications

Product Data
Clone Name	R04-5A4
Applications	IF, IHC, IP, WB
Recommend Dilution	WB: 1/1000-1/10000 IHC: 1/50 ICC/IF: 1/100 IP: 1/20
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal
Immunogen	A synthetic phosphopeptide corresponding to residues surrounding Ser209 of human eIF4E (Phosphorylated)
Isotype	IgG
Formulation	50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Concentration	lot specific
Purification	Affinity Purified
Conjugation	Unconjugated
Storage Condition	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Predicted Protein Size	Calculated MW: 25 kDa; Observed MW: 25 kDa
Gene Name	eukaryotic translation initiation factor 4E
Database Link	Entrez Gene 1977 Human UniProt ID P06730
Background	Swiss-Prot Acc.P06730.Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Synonyms	CBP; eIF-4E; EIF4E1; EIF4EL1; EIF4F; MGC111573
Reference Data

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