C13orf8 (CHAMP1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	WB: 200-1000 WB positive control: A172 cell lysate IHC: 25-100 Positive control: Human ovarian cancer Predicted cell location: Nucleus and Cytoplasm
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide of human CHAMP1
Isotype	IgG
Formulation	pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Concentration	lot specific
Purification	Antigen affinity purification
Conjugation	Unconjugated
Storage Condition	Store at -20°C.
Predicted Protein Size	89 kDa
Gene Name	chromosome alignment maintaining phosphoprotein 1
Database Link	Entrez Gene 283489 Human UniProt ID Q96JM3
Background	CHAMP1 (chromosome alignment maintaining phosphoprotein 1), also known as ZNF828 or C13orf8, is a 812 amino acid protein containing one C2H2-type zinc finger. CHAMP1 is required for the proper alignment of chromosomes during metaphase, undergoing CDK1-dependent phosphorylation at multiple sites during mitosis. The phosphorylation counteracts the negative chromosomal alignment regulation of the zinc-finger domain of CHAMP1. One region of CHAMP1, the FPE region, is responsible for spindle and kinetochore localization, which is essential for proper chromosome alignment. CHAMP1 interacts with MAD2L2, PGOZ, CBX1, CBX3 and CBX5, and may recruit CENPE and CENPF to the kinetochore. The CHAMP1 gene is located on chromosome 13 and is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat and chicken.
Synonyms	RGD1311283; Zfp828
Reference Data

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