Macrophages Mouse Monoclonal Antibody [Clone ID: ED3]

Specifications

Product Data
Clone Name	ED3
Applications	IHC
Recommend Dilution	Tested for immunohistochemistry (IHC); has been described to work in FACS. Approximate working dilution for IHC: Frozen sections: 2µg/ml (1:500) Paraffin sections: not tested. Optimal dilutions should be determined by the end user. Suggested positive control: Rat spleen.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Rat spleen cells.
Specificity	Rat: macrophages. Other species: not tested Epitope: ED 3 recognises a membrane protein which is associated with a receptor for glycoconjugates. Distribution: ED3 recognizes a membrane antigen on macrophages. Bone marrow-derived macrophages stimulated by T-cells are positive with ED 3.
Isotype	IgG2a
Formulation	Purified, liquid Supplied as a 0.25ml solution This stock solution contains 1mg/ml IgG, phosphate buffered saline pH 7.2 (PBS), no stabilizer and 0.09% sodium azide as a preservative.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Stock solution or aliquots thereof: 1 year at -20°C. Avoid repeated thawing and freezing.
Background	Monoclonal antibody ED 3 is a characteristic marker for tissue fixed macrophage subpopulations, particularly of lymphoid organs (e.g. marginal zone metallophilic macrophages). No other cell types, including monocytes and granulocytes, are positive. Macrophages in autoimmune diseased but not in healthy tissues express the ED3 antigen. The recognised antigen appears to be a receptor for sialylated glycoconjugates
Reference Data

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