Cd163 Mouse Monoclonal Antibody [Clone ID: ED2]

CAT#: TA363848

CD163 (scavenger receptor, haemoglobin-haptoglobin receptor), mouse anti rat, clone ED2



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CNY 9,600.00


货期*
5周

规格
    • 250 ug

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Specifications

Product Data
Clone Name ED2
Applications IHC
Recommend Dilution Tested for immunohistochemistry (IHC); has been described to work in FACS, immunprecipitation. Approximate working dilution for IHC: Frozen sections: 1µg/ml (1:1000) Paraffin sections: 20µg/ml (1:50 Proteinase K pretreatment for antigen retrieval is recommended Optimal dilutions should be determined by the end user. Suggested positive control: Rat spleen.
Reactivity Rat
Host Mouse
Clonality Monoclonal
Immunogen Rat spleen cells.
Specificity Rat: macrophages. Other species: not tested

Epitope: The antigen has been described as CD163, epitope not further characterized.

Distribution: Isolated cells: Monocytes and dendritic cells harvested from spleen and lymph nodes, and granulocytes harvested from the peritoneal cavity are negative with ED 2.
Formulation Affinity purified, liquid. Supplied as a 0.25ml solution. This stock solution contains 1mg/ml IgG, phosphate buffered saline pH 7.2 (PBS), no stabilizer and 0.09% sodium azide as a preservative.
Concentration lot specific
Conjugation Unconjugated
Storage Condition Stock solution or aliquots thereof: 1 year at -20°C. Avoid repeated thawing and freezing.
Gene Name CD163 molecule
Background Monoclonal antibody ED2 reacts with a membrane antigen (175, 160 and 95kDa) on resident rat macrophages. Monocytes, dendritic cells (spleen and lymph node), peritoneal granulocytes and other cell types are negative. ED2 discriminates between thymic cortical (positive for ED2) and medullary (negative for ED2) macrophages. It is useful to stain synovial lining cells and Kupffer cells in the liver. The antigen has recently been shown to be identical with CD163 (Polfliet, M.M.J. et al [2002], PhD Thesis, Vrije Universiteit, Amsterdam).
Synonyms M130; MM130
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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