HLA II Mouse Monoclonal Antibody [Clone ID: HKB1Biotin]

Specifications

Product Data
Clone Name	HKB1Biotin
Applications	IHC
Recommend Dilution	Tested for immunohistochemistry (IHC); has been described to work in FACS. Approximate working dilution for IHC: Frozen sections: 0.5-1µg/ml (1:400-1:800) Paraffin sections: not tested Optimal dilutions should be determined by the end user. Suggested positive control: Human tonsil
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	REH cells, a human B cell precursor leukemia cell line.
Specificity	Human: HLA II Other: not tested Epitope: The antigen is HLA II, the epitope has not been further characterized.
Formulation	Affinity purified, liquid Supplied as a 0.5ml solution. This stock solution contains 0.4mg/ml IgM, phosphate buffered saline pH 7.2 (PBS) and 0.05% (v/v) Kathon CG as a preservative.
Concentration	lot specific
Conjugation	Biotin
Storage Condition	Original vial: 1 year at 4° - 8°C. Stock solution or aliquots thereof: 1 year at -20°C. Avoid repeated thawing and freezing.
Background	Monoclonal antibody HKB1 recognizes all HLA class II antigens, the 3 major (HLA-DP, -DQ and -DR) and 2 minor Major Histocompatibility Complex MHC class II proteins (HLA-DM and -DO). The genes of the class II combine to form heterodimeric (αβ) protein receptors that are typically expressed on the surface of antigen presenting cells (APCs): dendritic cells B-cells and macrophages. HLA class II is presented on activated T-cells. HLA class II molecules present exogenously derived antigen to the T cell receptor (TCR) on CD4+ T lymphocytes.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.