PD-L1 (CD274) Mouse Monoclonal Antibody [Clone ID: 1F11]

Specifications

Product Data
Clone Name	1F11
Applications	IF, IHC, WB
Recommend Dilution	WB: 1-2µg/mL. IHC starting at 2-µg/mL. IF start at 5µg/mL.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	PD-L1 antibody was raised against the extracellular domain of human PD-L1.
Isotype	IgG1
Formulation	PD-L1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/ml
Purification	PD-L1 Antibody is supplied as protein A purified IgG1.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	Predicted: 32 kDa; Observed: 45 kDa
Gene Name	CD274 molecule
Database Link	NP_054862 Entrez Gene 29126 Human UniProt ID Q9NZQ7
Background	PD-L1 Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC) (1). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC (2). PD-1 can bind two different but related ligands, PD-L1 and PD-L2. PD-L1 is a B7-related protein that inhibits cell-mediated immune responses by reducing the secretion of IL-2 and IL-10 from memory T cells (3). This suggests that PD-L1 may be useful in reducing allogenic CD4+ memory T-cell responses to endothelial cells, thereby reducing the likelihood of host immune responses to allografts. PD-L1 also functions as an immune checkpoint protein, and multiple anti-PD-L1 antibodies are currently in phase II and III clinical trials, with one antibody already approved for the treatment of cancer (4).
Synonyms	B7-H; B7-H1; B7H1; MGC142294; MGC142296; PD-L1; PDCD1L1; PDCD1LG1; PDL1
Note	PD-L1 antibody can be used for detection of PD-L1 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2 - µg/mL. For immunofluorescence start at 5 µg/mL.
Reference Data

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