ROCK2 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
Reactivity	Human, Mouse, Rat, Chicken, Bovine, Canis
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide corresponding to the epitope -CDTAV- with a single phosphorylation site Thr249 of human Rho Kinase/RockII protein. This sequence is identical among human, mouse, rat, chicken, dog and bovine.
Isotype	IgG
Formulation	This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer.
Purification	The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	165 kDa
Gene Name	Rho associated coiled-coil containing protein kinase 2
Database Link	NP_001308572 Entrez Gene 19878 MouseEntrez Gene 25537 RatEntrez Gene 9475 Human UniProt ID O75116
Background	Rho A-dependent kinase (ROCKII), or ROK-alpha, is identified a serine/threonine kinase, a downstream effector of the small GTPase Rho, mediating effects of Rho on the actin cytoskeleton, formation of actin stress fibers and focal adhesions, ROCKII alters cardiac myofilament response to Ca2+ by mechanisms involving phosphorylation of thin filament proteins. ROCK-II induces a depression in maximum ATPase rate and tension, which is associated with phosphorylation of troponin T (TnT), troponin I (TnI), and myosin-binding protein C (C-protein). The phosphorylation of Rho-kinase at Thr 249, Thr 396 plays important role in G-protein signaling..
Synonyms	ROCK-II
Reference Data
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Axon guidance, Chemokine signaling pathway, Focal adhesion, Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, TGF-beta signaling pathway, Vascular smooth muscle contraction, Wnt signaling pathway

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