Adenosine A2b Receptor (ADORA2B) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, WB
Recommend Dilution	ADORA2B antibody can be used for detection of ADORA2B by Western blot at 0.25 – 0.5 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	ADORA2B antibody was raised against a 19 amino acid peptide near the carboxy terminus of human ADORA2B. The immunogen is located within the last 50 amino acids of ADORA2B.
Specificity	ADORA2B antibody is human, mouse and rat reactive. At least two isoforms of ADORA2B are known to exist; this antibody will detect both isoforms. ADORA2B antibody is predicted to not cross-react with ADORA2A.
Isotype	IgG
Formulation	PBS containing 0.02% sodium azide.
Concentration	1 mg/ml
Purification	ADORA2B antibody is affinity chromatography purified via peptide column.
Conjugation	Unconjugated
Storage Condition	Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Predicted Protein Size	Predicted: 37 kDa; Observed: 41 kDa
Gene Name	adenosine A2b receptor
Database Link	NP_000667 Entrez Gene 11541 MouseEntrez Gene 29316 RatEntrez Gene 136 Human UniProt ID P29275
Background	The adenosine receptor ADORA2B is a member of the G protein-coupled receptor superfamily and is an integral membrane protein that stimulates adenylate cyclase activity in the presence of adenosine (1,2). Extracellular adenosine triggers a potent anti-inflammatory response that is mediated in part by ADORA2B including the stimulation of IL-10 production (3-5). Activation of ADORA2B can also enhance the abundance of regulatory T cells (Tregs), a class of cells that work to constrain inflammation (6).
Synonyms	ADORA2
Reference Data
Protein Families	Druggable Genome, GPCR, Transmembrane
Protein Pathways	Calcium signaling pathway, Neuroactive ligand-receptor interaction, Vascular smooth muscle contraction

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