alpha Tubulin (TUBA3C) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	WB: 0.25 - 0.5 ug/mL, IHC: 5 ug/mL, IF: 20 ug/mL
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Alpha-tubulin antibody was raised against an 18 amino acid peptide near the amino terminus of human alpha-tubulin
Isotype	IgG
Formulation	PBS containing 0.02% sodium azide.
Concentration	1 mg/ml
Purification	Alpha-tubulin antibody is affinity chromatography purified via peptide column.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	Predicted: 50 kDa; Observed: 45 kDa
Gene Name	tubulin alpha 3c
Database Link	NP_005992 Entrez Gene 7278 Human UniProt ID Q13748
Background	Alpha-tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter (reviewed in 1). Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase (2). Another post-translational modification of detyrosinated alpha-tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle (3). Like GAPDH and ?-Actin, this antibody makes an excellent loading control in immunoblots.
Synonyms	SCA41; TRP3
Reference Data

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