TLR9 Mouse Monoclonal Antibody [Clone ID: 26C593.2]

Specifications

Product Data
Clone Name	26C593.2
Applications	Block/Neutralize, CyTOF-ready, Dot, ELISA, FC, ICC/IF, IHC, IP, WB
Recommend Dilution	Immunohistochemistry, Block/Neutralize, Knockdown Validated, Functional, In vitro assay, ELISA: 1:100 - 1:2000, Immunoprecipitation: 1:10 - 1:500, Flow (Intracellular): 1:10 - 1:1000, Dot Blot, Western Blot: 2-5 ug/ml, Immunohistochemistry-Paraffin: 5 ug/ml, Flow Cytometry, Immunocytochemistry/ Immunofluorescence: 1:10-1:500, Simple Western: 30 ug/ml, CyTOF-ready
Reactivity	Human, Mouse, Rat, Canine, Equine, Primate
Host	Mouse
Clonality	Monoclonal
Immunogen	This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 268-300 of human TLR9 isoform A (Genbank accession no. AAF78037).
Isotype	IgG1, kappa
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	toll like receptor 9
Database Link	NP_059138 Entrez Gene 81897 MouseEntrez Gene 338457 RatEntrez Gene 54106 Human UniProt ID Q9NR96
Synonyms	CD289
Note	Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. In human PBMC, a ~120 kDa band is observed. A smaller isoform, TLR9 isoform B (Genbank accession no. AAF72190) containing 975 amino acids may also be observed in some cases.
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Toll-like receptor signaling pathway

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