TrkC (NTRK3) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC
Recommend Dilution	Immunohistochemistry: 1:200, Immunocytochemistry/ Immunofluorescence: 1:1000, Immunohistochemistry-Paraffin: 1:200
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to the extracellular domain of the human TrkC protein (within residues 300-400). [UniProt Q16288].
Formulation	PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	neurotrophic receptor tyrosine kinase 3
Database Link	NP_002521 Entrez Gene 18213 MouseEntrez Gene 4916 Human UniProt ID Q16288
Background	Neurotrophic tyrosine kinase receptor type 3 (NTRK3), commonly known as TRKC, is a member of the protein kinase superfamily, the tyrosine (Tyr) protein kinase family, and more specifically, the insulin receptor subfamily. TRKC is a single pass type I membrane protein that phosphorylates itself (via ligand-mediated auto-phosphorylation) and other proteins in the MAPK pathway upon neurotrophin binding. Mutations to the TRKC gene have been correlated with the development of medulloblastomas, secretory breast carcinomas, and other cancers. TRKC has several transcript variants that encode different isoforms (A= 94.4kDa, B= 68.4kDa, C= 92.8kDa, and D= 93.3kDa), and each isoform has unique signaling properties. Cell differentiation that plays a role in the development of proprioceptive neurons originates via signaling through TRKC. The TRKC protein contains two Ig-like C2-type immunoglobulin-like domains, two leucine-rich repeats (LRR), one leucine rich repeat C-terminal (LRRCT) domain, and one protein kinase domain.
Synonyms	gp145(trkC); TRKC
Note	This TrkC antibody is useful for Immunohistochemistry-Paraffin and Immunocytochemistry / Immunofluorescence. In ICC/IF, membrane staining was observed in neuro2a cells. In IHC-P, staining was observed in the membrane of mouse brain. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reference Data
Protein Families	Druggable Genome, Protein Kinase, Transmembrane
Protein Pathways	Neurotrophin signaling pathway

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