GLS2 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, ICC/IF, IHC, Simple Western, WB
Recommend Dilution	ELISA: 1:100-1:2000, Immunohistochemistry: 5 ug/ml, Immunocytochemistry/ Immunofluorescence: 20 ug/mL, Western Blot: 0.5-1 ug/ml, Immunohistochemistry-Paraffin: 5 ug/ml, Simple Western: 1:100
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Antibody was raised against a 18 amino acid peptide near the center terminus of human GLS2 (NP_037399).
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Peptide affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	66 kDa
Gene Name	glutaminase 2
Database Link	NP_037399 Entrez Gene 192268 RatEntrez Gene 216456 MouseEntrez Gene 27165 Human UniProt ID Q9UI32
Background	Phosphate-activated glutaminase, also known as Glutaminase 2 (GLS2), was initially isolated from rat liver, although it has been shown to be expressed in other tissues. Like the functionally similar, larger kidney glutaminase, GLS2 catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Expression of GLS2 is increased by p53 under both stressed and nonstressed conditions, resulting in increased levels of glutamate and alpha-ketoglutarate, which in turn results in enhanced mitochondrial respiration and ATP generation. GLS2 also regulates antioxidant defense function in cells by increasing reduced glutathione levels and decreasing ROS-levels, suggesting that GLS2 acts as a mediator of p53's role in antioxidant defense in addition to its role in energy metabolism.
Synonyms	GA; GLS; hLGA; LGA
Reference Data
Protein Pathways	Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, D-Glutamine and D-glutamate metabolism, Metabolic pathways, Nitrogen metabolism

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