N Cadherin (CDH2) Mouse Monoclonal Antibody [Clone ID: 13A9]

Specifications

Product Data
Clone Name	13A9
Applications	FC, ICC, ICC/IF, IHC, IP, Simple Western, WB
Recommend Dilution	Immunocytochemistry/ Immunofluorescence: 1:100, Simple Western: 1:50, Western Blot: 0.5 ug/ml, Immunohistochemistry: 1:50-1:200, Immunohistochemistry-Paraffin: 1:50-1:100, Immunoprecipitation: 1:10-1:500, Flow (Intracellular), Flow Cytometry, Immunocytochemistry
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Cytoplasmic domain of human N Cadherin [Swiss-Prot# P19022]
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	140 kDa
Gene Name	cadherin 2
Database Link	NP_001783 Entrez Gene 12558 MouseEntrez Gene 83501 RatEntrez Gene 1000 Human UniProt ID P19022
Background	N-cadherin (neuronal cadherin; or CDH2/cadherin 2) is a calcium-binding, single pass transmembrane cell adhesion molecule (CAM) that was originally believed to be expressed only by neural cells, but subsequently demonstrated in endothelial cells and pericytes of microvessels, as well as in a variety of poorly differentiated carcinomas. N-cadherin is found in many different types of intercellular junctions, such as adherens junctions and pericyte-endothelial cell junctions, as well as being distributed on the cell surface in non-junctional complexes. It interacts with CDCP1 and forms a part of complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. It also Interact with PCDH8 and TAOK2, and the interaction with PCDH8 leads to internalization through TAOK2/p38 MAPK pathway. N-cadherin promotes the formation of stable intercellular junctions and in addition to mediating cell adhesion, it has several other functions such as activation of FGFR, promotion of vascular smooth muscle and cancer cell migration as well as neurite outgrowth on astrocytes, and contrastingly, inhibits Schwann cell migration on astrocytes. Alongwith vimentin, N-cadherin has emerged as an important marker of EMT in embryonic development and carcinogenic progression.
Synonyms	CD325; CDHN; CDw325; NCAD
Note	This N Cadherin (13A9) antibody is useful for Immunofluorescence/Immunocytochemistry, Immunoprecipitation, Immunohistochemistry on paraffin-embedded sections and Western Blot, where a band is observed at ~140 kDa.
Reference Data
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
Protein Pathways	Arrhythmogenic right ventricular cardiomyopathy (ARVC), Cell adhesion molecules (CAMs)

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.