PDE9A Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, IP, WB
Recommend Dilution	Immunohistochemistry: 1:400, Immunocytochemistry/ Immunofluorescence: 1:1000, Immunoprecipitation: 1:1000, Western Blot: 1:1000, Immunohistochemistry-Paraffin: 1:400
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Sequence from the C-terminal region of PDE9A
Formulation	PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	68 kDa
Gene Name	phosphodiesterase 9A
Database Link	NP_002597 Entrez Gene 18585 MouseEntrez Gene 191569 RatEntrez Gene 5152 Human UniProt ID O76083
Background	Cyclic nucleotide phosphodiesterases (PDEs) are enzymes which catalyzes the hydrolysis of cyclic adenosine 30,50-monophosphate (cAMP) and/or cyclic guanosine 30,50-monophosphate (cGMP); and PDE9A (high affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A) is a PDE member which hydrolyzes the second messenger cGMP, an important regulator of several critical cellular physiological processes. PDE9A activity gets modulated by metal ions and it can bind 2 divalent metal cations per subunit - site 1 binds zinc ions, whereas, site 2 preferentially associates with magnesium and/or manganese ions. PDE9A is expressed in most of the solid tissues and because of its location as well as contribution to the regulation of steady-state cellular concentrations of cyclic nucleotides, PDE9A has been suggested as a potential candidate for diseases such as bipolar affective disorder and its overexpression has been linked to Down syndrome. Moreover, PDE9A deficient mice fed a high-fat diet have been reported to exhibit a reduced weight gain and fat mass compared with wild-type mice.
Synonyms	HSPDE9A2
Note	This PDE9A antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections, Immunoprecipitation, and Western Blot, where a band can be seen at ~68 kDa. In ICC/IF, cytoplasmic staining was observed in NIH-3T3 cells.
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Progesterone-mediated oocyte maturation, Purine metabolism

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