DNMT3A Mouse Monoclonal Antibody [Clone ID: 64B1446]

CAT#: TA336580

Mouse Monoclonal DNMT3A Antibody (64B1446)



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CNY 6,188.00


货期*
5周

规格
    • 100 ug

Cited in 2 publications.
经常一起买 (3)
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Specifications

Product Data
Clone Name 64B1446
Applications ChIP, CyTOF-ready, FC, ICC/IF, IHC, WB
Recommend Dilution Western Blot, Flow Cytometry: 1 ug per million cells, Immunocytochemistry/ Immunofluorescence: 1:10-1:500, Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin: 5 ug/ml, Knockdown Validated: Validated for Knockdown from CiteAb, CyTOF-ready
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Immunogen This antibody was raised against bacteria expressed recombinant mouse Dnmt3a. The epitope was found to lie near the C-terminus (a.a. 705-908), see Chen et (2002) for details.
Formulation PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration lot specific
Purification Protein G purified
Conjugation Unconjugated
Storage Condition Store at -20°C as received.
Gene Name DNA (cytosine-5-)-methyltransferase 3 alpha
Background Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.
Synonyms DNMT3A2; M.HsaIIIA; TBRS
Note Western Blot: Detects a band of approximately 120 kDa (predicted molecular weight: 102 kDa). Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Use in chromatin immunoprecipitation reported in scientific literature (PMID 24623306)
Reference Data
Protein Families Druggable Genome
Protein Pathways Cysteine and methionine metabolism, Metabolic pathways
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (2)

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