SIAH1 Mouse Monoclonal Antibody [Clone ID: 8G7H12]

Specifications

Product Data
Clone Name	8G7H12
Applications	IP, WB
Recommend Dilution	Western Blot: 1:500, Immunoprecipitation: 1:10-1:500
Reactivity	Human, Rat, Drosophila, Porcine, Zebrafish, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide made to a C-terminal portion of the drosophila SINA protein (within residues 280-331). [Swiss-Prot# P21461]
Formulation	Tris-citrate/phosphate, pH 7, 0.1% Sodium azide. Store at 4C. Do not freeze.
Concentration	lot specific
Purification	Tissue culture supernatant
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	37 kDa
Gene Name	siah E3 ubiquitin protein ligase 1
Database Link	NP_003022 Entrez Gene 140941 RatEntrez Gene 6477 Human UniProt ID Q8IUQ4
Background	The Drosophila SINA (seven in absentia) protein is an E3 ubiquitin ligase component of the RAS signal transduction pathway. The RAS signal pathway controls cell proliferation, differentiation, and survival, and regulation of this pathway is critical for normal development. In Drosophila SINA serves as a downstream gatekeeper required for proper RAS signal transduction. Similarly to SINA in Drosophila, the human protein SIAH has also been shown to be required for oncogenic RAS signaling in cancer.
Synonyms	SIAH1A
Note	This SIAH1/2 antibody is useful for Immunoprecipitation and Western blot, where a band is seen at ~37 kDa.
Reference Data
Protein Families	Druggable Genome
Protein Pathways	p53 signaling pathway, Ubiquitin mediated proteolysis, Wnt signaling pathway

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