CD11b (ITGAM) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, ICC/IF, IHC, WB
Recommend Dilution	Flow Cytometry: 1:50 - 1:200, Immunocytochemistry/ Immunofluorescence: 1:50-1:200, Immunohistochemistry: 5-10 ug/ml, Immunohistochemistry-Paraffin: 5-10 ug/ml, Western Blot: 2-4 ug/ml, Immunohistochemistry-Frozen
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide made to an internal region (within residues 500-600) of the mouse CD11 (b/c) protein. [Swiss-Prot# P05555]
Formulation	Tris-glycine, 150 mM NaCl, 0.05% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	160 kDa
Gene Name	integrin subunit alpha M
Database Link	NP_000623 Entrez Gene 16409 MouseEntrez Gene 3684 Human UniProt ID P11215
Background	CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% of thymocytes and is commonly used as a microglial marker in nervous tissue.
Synonyms	CD11B; CR3A; MAC-1; MAC1A; MO1A; SLEB6
Note	This CD11b/c antibody is useful for Immunocytochemistry/Immunofluorescence, Western Blot, Immunohistochemistry paraffin embedded sections and Flow Cytometry. In Western blot a specific band is observed ~ 160 kDa and an apparant non-specific band is observed ~ 60 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with citrate buffer (pH 6.0) is recommended. In ICC/IF, membrane staining was observed in Raw 264.7 cells.
Reference Data
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
Protein Pathways	Cell adhesion molecules (CAMs), Hematopoietic cell lineage, Leukocyte transendothelial migration, Regulation of actin cytoskeleton

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.