TLR3 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	Dot, FC, IHC, WB
Recommend Dilution	Flow (Cell Surface)), Flow (Intracellular): 1 ug/1 million cells, Immunohistochemistry-Frozen: 1:20-1:1000, Immunohistochemistry-Paraffin: 1:100, Knockdown Validated, Dot Blot, Immunohistochemistry: 1:20-1:1000, Flow Cytometry: 1 ug/10^6 cells, Western Blot: 1-3 ug/ml
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	A mix of synthetic peptides corresponding to amino acids 135-150 (SIHKIKSNPFKNQKNL), 828-844 (CRRFKVHHAVQQAIEQN), and 876-891 (CILNWPVQKERINAFH) of mouse TLR3.
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	104 kDa
Gene Name	toll like receptor 3
Database Link	NP_003256 Entrez Gene 142980 MouseEntrez Gene 7098 Human UniProt ID O15455
Background	The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described. mTLR3 cDNA codes for a protein with approximate molecular weight of 100 kDa . TLR3 has a restricted expression pattern being expressed in dendritic cells (DC). TLR3 mRNA expression was detected by in situ hybridization in DC and lymph nodes. The expression of TLR3 in a single cell type may indicate a specific role for this molecule in a restricted setting.
Synonyms	CD283; IIAE2
Note	Flow Cytometry (Cell Surface): see Pawar et al. (2006) for details Flow Cytometry (Intracellular): 1 ug/10^6 cells Immunohistochemistry-Frozen: see Pwar et al, 2005 for details. Immunohistochemistry-Paraffin: 1:50 (see Patol et al, 2005 for details)
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Toll-like receptor signaling pathway

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.