DNMT1 Mouse Monoclonal Antibody [Clone ID: 60B1220.1]

Specifications

Product Data
Clone Name	60B1220.1
Applications	ChIP, CyTOF-ready, FC, ICC/IF, IHC, IP, Simple Western, WB
Recommend Dilution	Simple Western: 1:100, Flow (Intracellular), Immunoprecipitation: 1:10-1:500, Western Blot: 0.1-0.5 ug/ml, Immunohistochemistry: 1:50-1:500, Immunohistochemistry-Paraffin: 1:50-1:500, Flow Cytometry: 1 - 5 ug/mL, Chromatin Immunoprecipitation (ChIP): 1:20-1:1000, Immunohistochemistry-Frozen: 1:500, Immunohistochemistry Free-Floating: 1:500, Knockdown Validated, CyTOF-ready, Immunocytochemistry/ Immunofluorescence: 1:10
Reactivity	Human, Mouse, Zebrafish
Host	Mouse
Clonality	Monoclonal
Immunogen	This antibody was raised against a synthetic peptide corresponding to amino acids 637-650 (EKDDREDKENAFKR) of human Dnmt1 (Genbank Accession No. NP_001370).
Formulation	PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	DNA (cytosine-5-)-methyltransferase 1
Database Link	NP_001124295 Entrez Gene 13433 MouseEntrez Gene 1786 Human UniProt ID P26358
Background	Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells. NB100-56519 recognizes Dnmt1, a 1616 amino acid protein according to GenBank no. NP_001370.
Synonyms	ADCADN; AIM; CXXC9; DNMT; HSN1E; m.HsaI; MCMT
Note	Chromatin Immunoprecipitation, Immunoprecipitation, Western Blot and Immunohistochemistry-Paraffin. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24386225)
Reference Data
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	Cysteine and methionine metabolism, Metabolic pathways

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