HIF-1 alpha (HIF1A) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ChIP, FC, ICC/IF, IHC, Immunoblotting, IP, Simple Western, WB
Recommend Dilution	Immunohistochemistry: 5 - 10 ug/mL, Immunohistochemistry-Frozen: 5 - 10 ug/mL, Immunohistochemistry-Paraffin: 5 - 10 ug/mL, Immunocytochemistry/ Immunofluorescence: 1:100, Immunoprecipitation: 1:10 - 1:500, Flow Cytometry: 1:10 - 1:1000, Immunoblotting, Immunohistochemistry Whole-Mount, Simple Western, Chromatin Immunoprecipitation (ChIP): 1:10-1:500, Western Blot: 1-2ug/ml
Reactivity	Human, Mouse, Rat, Goat, Hamster, Rabbit, Primate
Host	Rabbit
Clonality	Polyclonal
Immunogen	A fusion protein including residues 530-825 of the mouse HIF-1 alpha protein. [UniProt# Q61221]
Formulation	PBS, 0.02% Sodium azide (preservative)
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	115 kDa
Gene Name	hypoxia inducible factor 1 alpha subunit
Database Link	NP_851397 Entrez Gene 15251 MouseEntrez Gene 29560 RatEntrez Gene 3091 Human UniProt ID Q16665
Background	HIF1 (hypoxia-inducible factor 1), a heterodimeric transcription factor complex central to cellular response to hypoxia, consists of two subunits (HIF-1 alpha and HIF-1 beta) which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family. Expression of HIF-1 alpha protein is regulated by cellular oxygen level alterations as well as in oxygen-independent manner via different cytokines (through the PI3K-AKT-mTOR pathway), growth factors, oncogenic activation, or loss of tumor suppressor function etc. In normoxic cells, HIF-1 alpha is proline hydroxylated leading to a conformational change that promotes its binding to the VLH (von Hippel Lindau) protein E3 ligase complex; ubiquitination and followed by rapid proteasomal degradation. Hypoxia as well as chemical hydroxylase inhibitors (desferrioxamine, cobalt etc.) inhibit HIF-1 alpha degradation and lead to its accumulation in the cells, whereas, contrastingly, HIF-1 beta/ARNT (AhR nuclear translocator) remains stable under both conditions. Besides their critical role in hypoxic response, HIF1s regulates the transcription of genes responsible for angiogenesis, erythropoiesis/iron-metabolism, glucose metabolism, cell proliferation/survival, adipogenesis, carotid body formation, B lymphocyte development and immune reactions.
Synonyms	bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8
Note	This HIF-1 alpha antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded and frozen sections and Western blot, recognizing a band in hypoxic samples at ~115 kDa. Rat retina and brain cortex have been tested. Rat brain cortex is a bit dirty but this antibody does detect upregulation of HIF-1 alpha in hypoxic samples. HIF-1 alpha Protein NBC1-18422 has been successfully used for antibody preadsorption/blocking assay in IHC-P with Hif-1alpha antibody NB100-479 (refer to product review by Yves Heremans on the datasheet of NBC1-18422).
Reference Data
Protein Families	Transcription Factors
Protein Pathways	mTOR signaling pathway, Pathways in cancer, Renal cell carcinoma

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