IRE1 (ERN1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ICC/IF, IHC, WB
Recommend Dilution	Immunocytochemistry/ Immunofluorescence: 1:100 - 1:250, Knockdown Validated, Western Blot: 1:1000 - 1:2000, Immunohistochemistry: 1:250 - 1:500, Immunohistochemistry-Paraffin: 1:250 - 1:500, Knockout Validated
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic peptide within the human IRE1 alpha protein (within residues 700-800). [Swiss-Prot #O75460]
Formulation	Tris-glycine, 150 mM NaCl, 0.05% Sodium Azide. Store at -20C. Avoid freeze-thaw cycles.
Concentration	lot specific
Purification	Immunogen affinity purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	110 kDa
Gene Name	endoplasmic reticulum to nucleus signaling 1
Database Link	NP_001424 Entrez Gene 78943 MouseEntrez Gene 498013 RatEntrez Gene 2081 Human UniProt ID O75460
Background	Unfolded protein response (UPR) signaling, mechanism used by eukaryotic cells to cope ER stress, is initiated by three ER-localized protein sensors: PERK (PKR-like ER kinase), ATF (activating transcription factor 6), and IRE1 alpha (inositol-requiring enzyme 1 alpha). UPR-responsive genes's transcriptional activation is regulated by ATF6 and IRE1-XBP1 pathways, and UPR serves three important functions: inhibition of protein translation to restore normal cell functions; activation of signaling to increase production of molecular chaperones involved in protein folding; and activation of signaling that leads to targeting of misfolded proteins in ER for ubiquitination and subsequent degradation; or when ER-stress is not relieved, UPR leads to apoptosis. Localized as a single-pass type I membrane protein in ER membrane, IRE1 alpha is ubiquitously expressed with high levels observed in pancreatic tissue. It undergoes autophosphorylation and gets ADP-ribosylated by PARP16 upon ER stress, which increases its kinase as well as endonuclease activities. IRE1 alpha senses unfolded proteins in the ER lumen via its N-terminal domain which leads to enzyme auto-activation and thereafter, the active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent UPR transcriptional activator. IRE1 alpha inactivation in mice has been shown to result in widespread developmental defects, leading to intra-gestational embryonic lethality and ER stress mediated cell death is responsible for several pathologies.
Synonyms	hIRE1p; IRE1; IRE1a; IRE1P
Note	This IRE1 alpha antibody is useful for Western blot, where a band ~110 kDa is observed. In ICC/IF endoplasmic reticulum staining was observed in HeLa cells.
Reference Data
Protein Families	Protein Kinase, Transmembrane
Protein Pathways	Alzheimer's disease

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