ATM Rabbit Polyclonal Antibody

CAT#: TA336258

Rabbit Polyclonal ATM Antibody



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CNY 5,381.00


货期*
5周

规格
    • 50 ul

经常一起买 (1)
beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control
    • 30 ul

CNY 300.00
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Specifications

Product Data
Applications ELISA, FC, ICC/IF, IHC, Immunoblotting, IP, WB
Recommend Dilution Western Blot: 1:500-1:1000, Immunoblotting, Immunoprecipitation: 1:10-1:500, Immunocytochemistry/ Immunofluorescence: 1:100, Immunohistochemistry: 1:100, Immunohistochemistry-Paraffin: 1:100, ELISA, Flow Cytometry
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Immunogen A fragment of the human ATM protein corresponding to the C-terminus (within the last third of the protein sequence). [UniProt# Q13315]
Formulation PBS, 50% glycerol, 0.05% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Concentration lot specific
Purification Immunogen affinity purified
Conjugation Unconjugated
Storage Condition Store at -20°C as received.
Predicted Protein Size 350 kDa
Gene Name ATM serine/threonine kinase
Background ATM (ataxia telangiectasia mutated kinase), the master regulator of DNA double-strand breaks (DSBs) repair pathway, is a serine/threonine protein kinase that act as DNA damage sensor by activating checkpoint signaling upon DSBs, apoptosis and genotoxic stresses. ATM activation involves its recruitment to DSBs through interaction with the MRE11-RAD50-NBS1 or MRN complex, followed by KAT5/TIP60 mediated acetylation. ATM recognizes the substrate consensus sequence [ST]-Q and phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at DSBs, thereby regulating DNA damage response mechanism. ATM also implicates in vesicle and/or protein transport, T-cell development, gonads/neurological function, pre-B cell allelic exclusion, signal transduction, cell cycle control and act as a tumor suppressor. ATM exist as dimers or tetramers in inactive state and on DNA damage, autophosphorylation dissociates ATM into monomers rendering them catalytically active and binds/activates ABL1, SAPK, DYRK2, CHEK2, p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. ATM is an integral part of BASC complex (BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBN protein complex) and interacts with DCLRE1C, KAT8, KAT5, NABP2, ATMIN, CEP164, AP2B1, AP3B2, TELO2, TTI1, DDX1 etc. NUAK1/ARK5 mediated ATM phosphorylation and ATM autophosphorylation at Ser-367, Ser-1893, Ser-1981 correlates with DNA damage-regulated activation of the kinase. Defects in ATM are the cause of ataxia telangiectasia (AT), T-cell acute lymphoblastic leukemia (TALL) and T-prolymphocytic leukemia (TPLL), B-cell non-Hodgkin lymphomas (BNHL), including mantle cell lymphoma (MCL), B-cell chronic lymphocytic leukemia (BCLL).
Synonyms AT1; ATA; ATC; ATD; ATDC; ATE; TEL1; TELO1
Note This ATM antibody can be used for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections, Immunoprecipitation and Western Blot. In WB, it detects a band at ~350 kDa, representing ATM.
Reference Data
Protein Families Druggable Genome, Protein Kinase, Transcription Factors
Protein Pathways Apoptosis, Cell cycle, p53 signaling pathway
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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