HIF-1 alpha (HIF1A) Mouse Monoclonal Antibody [Clone ID: ESEE122]

Specifications

Product Data
Clone Name	ESEE122
Applications	IHC, WB
Recommend Dilution	IHC: 1:1000-1:8000, IF/ICC: 8-21ug/ml
Reactivity	Human, Mouse, Rat, Cow
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant fragment corresponding to amino acids 329-530
Isotype	IgG1
Formulation	PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration	lot specific
Purification	Protein G Purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	hypoxia inducible factor 1 alpha subunit
Database Link	NP_001521 Entrez Gene 15251 MouseEntrez Gene 29560 RatEntrez Gene 3091 Human UniProt ID Q16665
Background	Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response of hypoxia . The HIF1 complex consists of two subunits, HIF1-Alpha and HIF1-Beta, which are basic helix-loop-helix proteins of the PAS family . HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1 is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1 is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows . Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1 degradation and lead to its stabilization. In addition, HIF-1 can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway .
Synonyms	bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8
Note	Specific for HIF1Alpha
Reference Data
Protein Families	Transcription Factors
Protein Pathways	mTOR signaling pathway, Pathways in cancer, Renal cell carcinoma

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