GRP94 (HSP90B1) Rat Monoclonal Antibody [Clone ID: 9G10]

Specifications

Product Data
Clone Name	9G10
Applications	WB
Recommend Dilution	0.5ug/ml was sufficient for detection of Grp94 in 20ug of HeLa lysate.
Reactivity	Human, Mouse, Rat, Bovine, Canine, Chicken, Hamster, Rabbit, Monkey, Pig, Sheep, Xenopus, Horse, Guinea Pig
Host	Rat
Clonality	Monoclonal
Immunogen	Purified Grp94 isolated from chicken oviducts
Isotype	IgG2a
Formulation	PBS pH7.2, 50% glycerol, 0.09% sodium azide
Concentration	lot specific
Purification	Protein G Purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	heat shock protein 90kDa beta family member 1
Database Link	NP_003290 Entrez Gene 22027 MouseEntrez Gene 362862 RatEntrez Gene 699568 MonkeyEntrez Gene 7184 Human UniProt ID P14625
Background	Grp94 (glucose regulated protein 94, gp96) is a constitutively expressed endoplasmic reticulum (ER) lumenal protein that is up-regulated in response to cellular stress such as heat shock, oxidative stress or glucose depletion. Grp94 is thought to play a role in protein translocation to the ER, in their subsequent folding and assembly, and in regulating protein secretion . Grp94 also plays a role in antigen presentation by accessing the endogenous pathway and eliciting specific CTL responses to chaperone bound peptides via MHC class I pathway Grp94 is a member of the Hsp90 family of stress proteins and shares sequence homolgy with its cytosolic equivalent, Hsp90 . Both Hsp90 and Grp94 are calcium binding proteins . Despite sharing 50% sequence homology over its N domains and complete conservation in its ligand binding domains with Hsp90, Grp94 and Hsp90 differ in their interactions with regulatory ligands as Grp94 has weak ATP binding and hydrolyisis activity . Grp94 exists as a homodimer and the two subunits interact at two distinct intermolecular sites, C terminal dimerization domains and the N-terminal interacts with the middle domain of opposing subunits. . Grp94 contains a carboxy terminal KDEL (Lys-Asp-Glu-Leu) sequence which is believed to aid in its retention in the ER .
Synonyms	ECGP; GP96; GRP94; HEL-S-125m; HEL35; TRA1
Note	Detects a 98kDa protein corresponding to the molecular mass of Grp94 on SDS PAGE immunoblots. Does not detect human Hsp90, Grp74, or GrpE from E.coli.
Reference Data
Protein Families	Druggable Genome
Protein Pathways	NOD-like receptor signaling pathway, Pathways in cancer, Prostate cancer

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