APAF1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	WB: 500-2000 WB positive control: Hela and A549 cells IHC: 25-100 Positive control: Human thyroid cancer Predicted cell location: Cytoplasm
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide corresponding to a region derived from 11-25 amino acids of Human Apoptotic protease-activating factor 1
Isotype	IgG
Formulation	PBS pH7.3, 0.05% NaN3, 50% glycerol
Concentration	lot specific
Purification	Antigen affinity purification
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	38 kDa
Gene Name	apoptotic peptidase activating factor 1
Database Link	NP_863659 Entrez Gene 11783 MouseEntrez Gene 78963 RatEntrez Gene 317 Human UniProt ID O14727
Background	This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain; a caspase recruitment domain (CARD); and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP; this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein; releasing its mature; activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.
Synonyms	APAF-1; CED4
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Huntington's disease, p53 signaling pathway, Parkinson's disease, Small cell lung cancer

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