Ahsa1 Rat Monoclonal Antibody [Clone ID: 25F2.D9]

Specifications

Product Data
Clone Name	25F2.D9
Applications	IHC, WB
Recommend Dilution	ELISA: 1:20,000, WB: 1:1,000, IHC: 5-10 ug/mL
Reactivity	Mouse, Human, Chimpanzee
Host	Rat
Clonality	Monoclonal
Immunogen	This Protein G purified monoclonal antibody was produced in rats by repeated immunizations with full length recombinant mouse AHA1 protein followed by hybridoma development.
Formulation	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	AHA1, activator of heat shock protein ATPase 1
Database Link	NP_666148 Entrez Gene 10598 HumanEntrez Gene 217737 Mouse UniProt ID Q8BK64
Synonyms	AHA1; C14orf3; p38
Note	Activator of Hsp90 ATPase (AHA1) stimulates the inherent ATPase cycle of Hsp90, which is essential for its chaperone activity in vivo. The activation and/or stability of many of the key regulatory and signaling proteins of the eukaryotic cell depend on their interaction with the Hsp90 molecular chaperone. Hsp90 is assisted and regulated by co-chaperones that participate in an ordered series both to assist client-protein recruitment or release and to modulate progress through the ATPase coupled chaperone cycle. Structural analysis and mutagenesis show that binding of the N-terminal domain of AHA1 to Hsp90 promotes a conformational switch in the middle-segment catalytic loop (aa 370–390) of Hsp90 that exposes the catalytic Arg380 and enables its interaction with ATP in the N-terminal nucleotide-binding domain of the chaperone. Recent studies show that AHA1 modulates Hsp90-dependent stability of the folding of the cystic fibrosis transmembrane conductance regulator (CFTR) in the endoplasmic reticulum (ER). Down-regulation of AHA1 rescues misfolding of CFTR in cystic fibrosis.
Reference Data

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