WHIP (WRNIP1) Goat Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	WB 1-3ug/ml; IHC 4-6ug/ml
Reactivity	Human, Mouse, Rat, Dog, Cow
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence EELRGVDFFKQRRC, from the C Terminus of the protein sequence according to NP_064520.2; NP_569079.1.
Formulation	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20C. Minimize freezing and thawing.
Concentration	lot specific
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	Werner helicase interacting protein 1
Database Link	NP_064520 Entrez Gene 78903 MouseEntrez Gene 282835 RatEntrez Gene 478698 DogEntrez Gene 56897 Human UniProt ID Q96S55
Synonyms	bA420G6.2; WHIP
Note	WB: Approx 90kDa band observed in lysates of cell line Jurkat (calculated MW of 72.1kDa according to NP_064520.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Bish RA, Myers MP., J Biol Chem. 2007 Aug 10;282(32):23184-93; PMID: 17550899). An additional band of unknown identity was also consistently observed at 50kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC: In paraffin embedded Human Kidney shows nuclear staining, stronger in DCT compared to PCT.
Reference Data

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