CIDE B (CIDEB) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, WB
Recommend Dilution	WB: 1-2 µg/mL IHC-P/IF: 5-20 µg/mL. Antibody validated: Western Blot in human, mouse and rat samples Immunofluorescence in the human, mouse and rat samples. All other applications and species not yet tested.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Anti-CIDE-B antibody (2319) was raised against a peptide corresponding to 15 amino acid synthetic peptide near the carboxy terminus of human CIDE-B. The immunogen is located within the last 50 amino acids of CIDE-B.
Specificity	CIDE-B antibody has no cross activity to CIDE-A.
Isotype	IgG
Formulation	CIDE-B Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/ml
Purification	CIDE-B Antibody is affinity chromatography purified via peptide column.
Conjugation	Unconjugated
Storage Condition	CIDE-B antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Predicted Protein Size	Predicted: 25 kD Observed: 26 kD
Gene Name	cell death-inducing DFFA-like effector b
Database Link	AF218586 Entrez Gene 27141 Human UniProt ID Q9UHD4
Background	CIDE-B Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. DFF45/ICAD has been identified as inhibitor of caspase activated DNase DFF40/CAD. DFF45 related proteins CIDE-A and CIDE-B (for cell death-inducing DFF-like effector A and B) were recently identified. CIDE contains a new type of domain termed CIDE-N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD. Expression of CIDE-B induces apoptosis, which is inhibited by DFF45. CIDE-B is a DFF45-inhibitable effector that promotes cell death and DNA fragmentation. CIDE-B is expressed mainly in liver and at lower levels in spleen, kidney, peripheral blood lymphocytes and bone marrow.
Synonyms	cell death-inducing DFFA-like effector b
Note	Optimal dilutions for each application to be determined by the researcher.
Reference Data

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