Nephrin (NPHS1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	WB: 1-4 µg/mL IHC-P: 1-2 µg/mL. Antibody validated: Western Blot in human, mouse and rat samples Immunohistochemistry in mouse, and rat samples. All other applications and species not yet tested.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Anti-Nephrin antibody (2265) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human Nephrin. The immunogen is located within the last 50 amino acids of Nephrin.
Isotype	IgG
Formulation	Nephrin Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Purification	Nephrin Antibody is affinity chromatography purified via peptide column.
Conjugation	Unconjugated
Storage Condition	Nephrin antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Predicted Protein Size	Predicted: 135 kD + 10 N-linked glycosylation sites Observed: 200 kD
Gene Name	NPHS1 nephrin
Database Link	NP_004637 Entrez Gene 54631 MouseEntrez Gene 64563 RatEntrez Gene 4868 Human UniProt ID O60500
Background	Nephrin Antibody: Nephrin is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth. Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm.
Synonyms	CNF; nephrin; NPHN
Note	Optimal dilutions for each application to be determined by the researcher.
Reference Data
Protein Families	Druggable Genome, Transmembrane

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