XBP1 Goat Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	ELISA: 1:4,000. WB: 1-3µg/ml.
Reactivity	Human, Mouse, Rat, Dog
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence C-NHSWEDTFANELFPQ, from the internal region of the protein sequence according to NP_001073007.1.
Formulation	Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration	lot specific
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Predicted Protein Size	28.5 kDa
Gene Name	X-box binding protein 1
Database Link	NP_005071 Entrez Gene 22433 MouseEntrez Gene 289754 RatEntrez Gene 477532 DogEntrez Gene 7494 Human UniProt ID P17861
Background	This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5. [provided by RefSeq]
Synonyms	TREB-5; TREB5; XBP-1; XBP2
Reference Data
Protein Families	Transcription Factors

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