Caspase 1 (CASP1) (C-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	Western blot: 0.5 µg - 1.0 µg/ml. Immunohistochemistry on paraffin sections: 0.5 µg - 1.0 µg/ml; This product does not require protein digestion pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining. Recommended Positive Control: Human heart tissue.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	A 16 amino acid peptide located near human Caspase-1 carboxy terminus.
Specificity	This antibody recognises an epitope within the C-terminal region (CT) of Caspase-1, otherwise known as IL-1Beta converting enzyme. This antibody detects a cleaved subunit band of approximately 21 kDa in heart cell lysates (predicted precursor MWT 45.2kDa).
Formulation	PBS containing 0.02% Sodium Azide State: Purified State: Liquid purified IgG
Concentration	lot specific
Purification	Affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	caspase 1
Database Link	Entrez Gene 834 Human UniProt ID P29466
Background	Caspase-1 is an intracellular cysteine protease, identified as a mammalian homologue to C. elegans cell death gene (ced-3). Caspase-1 has been classified as an inflammatory, rather than apoptotic caspase, due to its essential role in the cleavage of the inactive precursors of the cytokines IL-1beta and IL-18, into their mature activated and secretable forms. This regulation of pro-inflammatory cytokines by Caspase-1 has made inhibitors of Caspase-1 a possible target for use as therapeutic drugs for the treatment of inflammatory diseases.
Synonyms	CASP-1, CASP1, IL1BC, IL1BCE, ICE, p45
Reference Data

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